DNA sequencing with Python

Hello,
i need to add a step into a script (find attached).

The step is DNA sequencing with a Python library, for example:

http://biopython.org/DIST/docs/tutorial/Tutorial.html
https://github.com/souravsanyal06/DNA-Sequence-Alignment
https://github.com/sanger-pathogens/Fastaq#usage

You can find many on Github.

Thanks