Expert in Molecular Biology/Genetics Needed- Interpretation NGS pathways of Necrosis Apoptosis wound healing mouse reads of xenograft
Budget: $30 – $250 USD
Hello freelancers. The purpose of this project is to explore the changes in the host ( mouse) mammary tissue in a human breast xenograft during tumor progression and after experimental treatment, RNA seq. was performed. Gene expression reads of mouse for three pathways, Necrosis, Apoptosis and Wound healing were tabulated. I need interpretation of this data and comparison with known data. A small sample of the data is provided.
Explanation: My project is to understand what happens to the host cells when a tumor is implanted in their body.I had attached a screen shot of the data which lists 6 mouse( host) genes related to necrosis( Human breast cancer xenograft) .Each with 4 reads. The first column has the name of the gene and next 4 columns are the reads of the gene expression, the first 2 are pre-treatment and the last 2 are post treatment. Filters were set to only yield inverse regulation between growing and shrinking phase of the tumor. The entire data set for necrosis related genes is 23. Please convince me that you have the expertise in this field by make some observations and comments on the 6 listed genes expression reads for me to consider hiring you.
Explanation: My project is to understand what happens to the host cells when a tumor is implanted in their body.I had attached a screen shot of the data which lists 6 mouse( host) genes related to necrosis( Human breast cancer xenograft) .Each with 4 reads. The first column has the name of the gene and next 4 columns are the reads of the gene expression, the first 2 are pre-treatment and the last 2 are post treatment. Filters were set to only yield inverse regulation between growing and shrinking phase of the tumor. The entire data set for necrosis related genes is 23. Please convince me that you have the expertise in this field by make some observations and comments on the 6 listed genes expression reads for me to consider hiring you.